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When researchers want to focus on a primary gene of interest the use of antibiotic-resistance genes is extremely effective. Antibiotic-resistance genes are used as markers to identify the transfer of other genetic material (DNA) from one organism to another. An antibiotic-resistance gene, when used as a marker, produces a protein that protects plants and organisms from a specific antibiotic. When transformed cells (cells with an antibiotic-resistance gene) and untransformed cells are exposed to an antibiotic, such as G-418, only the transformed cells survive and grow. Researchers can therefore be confident that the surviving cells contain the gene of their primary research interest.
A.G. Scientific, Inc offers to researchers G418, among other antibiotic products. An aminoglycoside, G418, is used as a selective agent of transfected mammalian, yeast, plant and bacteria cells. Resistance is conferred by the bacterial gene for aminoglycoside-3'-phophotransferase that can be expressed in eukaryotic cells. Working concentrations of G418 range from 100 µg-5mg/ml. A concentration of ~400 µg/ml is needed for selection and ~200 µg/ml for maintenance in mammalian cells.
As a leading OEM supplier and the larges producer of Hygromycin B worldwide, we provide: